TET3, FLAG-Tag, His-Tag Recombinant

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Encompassing Amino Acids: 824-1795 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Description: Human Ten-eleven translocation 3 protein (TET3), also known as methylcytosine dioxygenase TET3, GenBank Accession # NM_001287491, a.a. 824-1795 (end) with N-terminal FLAG-tag and C-terminal His-tag. MW = 108 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, and 140 mM Imidazole

Genbank: NM_001287491

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Tags: N-terminal FLAG-tag, C terminal His-tag

Uniprot: O43151

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Tian-Peng, G., et al., Nature. 2011;477: 606-610.2. Abdel-Wahab, O., et al., Blood. 2009; 114: 144-147.

• Artikelnummer: BPS50163
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50163
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Wirt: Insect