Target Synonym: TFEB;ALPHATFEB;BHLHE35;TCFEB
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'; efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity.
Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Immunogen: Recombinant fusion protein of human TFEB
Dilution: WB 1:500-1:1000
Calculated MW: 43kDa/52kDa
Observed MW: 65kDa
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