TGFβ1, Latent Recombinant

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Encompassing Amino Acids: 30-390 (end)

Application: Useful for cell culture and for the study of signaling and apoptotic pathways.

Biological Activity: The ED50 measured by TGFβ1's ability to inhibit mIL-4-dependent proliferation of HT-2 mouse helper T cells is <0.5ng/ml.

Description: Human TGFbeta1 (GenBank Accession No. NM_000660), a.a. 30-390 (end) with Cys33Ser mutation and N-terminal His-tag. Mature latent TGFbeta1 consists of disulfide-linked homodimers of TGFbeta1 latency-associated peptide (LAP) and mature TGFbeta1, MW= ~75 & 25 kDa under non-reducing conditions.

Endotoxin Level: < 1EU per µg

Format: lyophilized protein

Formulation: Lyophilized from a 0.2 µm filtered, aqueous solution

Genbank: NM_000660

Host Cell Line: FreeStyle 293-F cells

Reconstitution: Reconstitute in sterile 0.2 M acetic acid with 0.1% BSA. Further dilutions may be made in PBS with 0.1% BSA. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage Stability: >6 months at -80°C (as a solid). Store reconstituted protein in aliquots at -80°C for up to 3 months. Avoid freeze/thaw cycles.

Tags: none

Uniprot: P01137

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Derynck, R. et al. (1985). Nature 316(6030):701-5.
2. Bourdrel L., et al. (1993). Protein Expr. Purif. 4:130-140.
3. Munger, J.S., et al. (1997). Kidney Int. 51:1376-1382.

• Artikelnummer: BPS90901-3
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90901-3
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Wirt: Human