Tie2, GST-tag Recombinant

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Encompassing Amino Acids: 771-1124(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 2 µM substrate (Tyr Peptide 5, Z-lyte kinase assay kit from Invitrogen), and enzyme at 37°C for 1 hour.

Description: Human TEK tyrosine kinase, endothelial, also known as Tie2, (GenBank Accession No. NM_000459) a.a. 771-1124(end) with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol

Genbank: NM_000459

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q02763

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Murray, B.W., et al., Biochemistry. 2001 Aug 28,40(34):10243-53.
2. Yuan, H.T., et al., Mol Cell Biol. 2009 Apr,29(8):2011-22.

• Artikelnummer: BPS40370
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40370
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect