Tie2, His-tag Recombinant

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Encompassing Amino Acids: 771-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 2 µg/ml recombinant Tie2.

Description: Human Tie2 (GenBank Accession No. NM_000459), a.a. 771-end with N-terminal His-tag, MW=41 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Genbank: NM_000459

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: Q02763

Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ramsden, J.D., et al., Endocrinology 146 (7), 2895-2902 (2005).
2. Tammela,T., et al., Blood 105 (12), 4642-4648 (2005).

• Artikelnummer: BPS40270
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40270
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect