TL1A-Responsive Luciferase Reporter Jurkat Cell Line

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Applications: Characterize the activity of soluble TL1A.Screen anti-TL1A antibodies in a cell-based assay format.

Background: TNF-like ligand 1A (TL1A, also known as Vascular endothelial growth inhibitor, or VEGI) is an anti-angiogenic cytokine. It is an important mediator of inflammation, participates in innate and adaptive immune homeostasis through binding to its receptor, DR3, and activating downstream signaling. Numerous studies showed that soluble TL1A can be detected in the serum of patients with T-cell mediated autoimmune diseases like rheumatoid arthritis, psoriatic arthritis, and inflammatory bowel disease. In addition, recent clinical studies suggested that anti-TL1A antibody treatment is a promising therapeutic approach in inflammatory disorders.  

Description: TNF-like ligand 1A (TL1A)-Responsive Luciferase Reporter Jurkat Cell Line is a TL1A-responsive DR3/NF-kB luciferase reporter Jurkat cell line expressing firefly luciferase under the control of an NF-kB response element, and with stable expression of human DR3 (death receptor 3; TNFRSF25; NM_003790.3). Expression of the firefly luciferase gene is driven by NF-kB response elements located upstream of the minimal TATA promoter. Activation of the NF-kB signaling pathway by DR3 ligand TL1A (TNF-like Protein 1A; TNFSF15) can be monitored by measuring luciferase activity. This cell line has been validated for stimulation by TL1A.

Host Cell Line: Jurkat cells (clone E6-1), Human T lymphoblast, suspension

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

• Artikelnummer: BPS78811
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78811
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human