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TMEM16A-HEK293 Recombinant Cell Line
TMEM16A-HEK293 Recombinant Cell Line
Artikelnummer
BPS90230
Verpackungseinheit
2 vials
Hersteller
BPS Bioscience
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Applications: Drug compound screening for activators or inhibitors of TMEM16A
Functional assays for TMEM16A
Efficient antigen for mouse immunization
Background: Calcium-activated chloride channels (CaCCs) are major regulators in numerous physiological processes including sensory transduction, epithelial secrection, cardiac and neutonal excitation, and smooth muscle contraction. TMEM16A (ANO1), a member of a family of putative plasma membrane proteins, is identified as a CaCC that is activated by intracellular Ca2+ and Ca2+-mobilizing stimuli. It has eight putative transmembrane segments without domains evidently involved in calcium regulation. The relative permeability of TMEM16A to monovalent anions is NO3–> I–>Br–> Cl– > F–.
Biological Activity: N-terminal FLAG tagged human TMEM16A channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The CaCC activity of TMEM16A was characterized by an assay based on halide-sensitive yellow fluorescent protein (YFP) mutant whose fluorescence is quenched by increasing halide concentration. When TMEM16A-expressed HEK293 cells were stimulated with ionomycin to raise the intracellular level of Ca2+, TMEM16A produced I- influx in HEK293 that triggered the rapid decreaseof fluorescence of transfected YFP mutant. The ionomycin-induced I- influx through TMEM16A was blocked by niflumic acid, a CaCC channel blocker.
Description: Stable, recombinant HEK293 cell line expressing human TMEM16A (transmembrane protein 16A, also called anoctamin 1, calcium-activated chloride channel (ANO1), accession number NM_018043).
Genbank: NM_018043
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~1 X 10^6 cells in 1 ml of 10% DMSO.
Uniprot: Q5XXA6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature Reviews 8: 153-171.
2. Caputo A. et al. (2008). Science 322: 590-594.
3. Yang Y.D. et al. (2008). Nature 455: 1210-1215.
Applications: Drug compound screening for activators or inhibitors of TMEM16A
Functional assays for TMEM16A
Efficient antigen for mouse immunization
Background: Calcium-activated chloride channels (CaCCs) are major regulators in numerous physiological processes including sensory transduction, epithelial secrection, cardiac and neutonal excitation, and smooth muscle contraction. TMEM16A (ANO1), a member of a family of putative plasma membrane proteins, is identified as a CaCC that is activated by intracellular Ca2+ and Ca2+-mobilizing stimuli. It has eight putative transmembrane segments without domains evidently involved in calcium regulation. The relative permeability of TMEM16A to monovalent anions is NO3–> I–>Br–> Cl– > F–.
Biological Activity: N-terminal FLAG tagged human TMEM16A channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The CaCC activity of TMEM16A was characterized by an assay based on halide-sensitive yellow fluorescent protein (YFP) mutant whose fluorescence is quenched by increasing halide concentration. When TMEM16A-expressed HEK293 cells were stimulated with ionomycin to raise the intracellular level of Ca2+, TMEM16A produced I- influx in HEK293 that triggered the rapid decreaseof fluorescence of transfected YFP mutant. The ionomycin-induced I- influx through TMEM16A was blocked by niflumic acid, a CaCC channel blocker.
Description: Stable, recombinant HEK293 cell line expressing human TMEM16A (transmembrane protein 16A, also called anoctamin 1, calcium-activated chloride channel (ANO1), accession number NM_018043).
Genbank: NM_018043
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~1 X 10^6 cells in 1 ml of 10% DMSO.
Uniprot: Q5XXA6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature Reviews 8: 153-171.
2. Caputo A. et al. (2008). Science 322: 590-594.
3. Yang Y.D. et al. (2008). Nature 455: 1210-1215.
| Artikelnummer | BPS90230 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 90230 |
| Verpackungseinheit | 2 vials |
| Mengeneinheit | PAK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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