TNKS2 (PARP5B) Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Contraindications: The TNKS2 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.

Description: The TNKS2 Chemiluminescent Assay Kit is designed to measure the activity of Tankyrase 2 (TNKS2, also known as PARP5B) for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS2 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: The TNKS2 assay kit comes in a convenient 96-well format, with purified TNKS2 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.

Uniprot: Q9H2K2

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS78406
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78406
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK