Target Synonym: TP53BP1; 53BP1; TDRD30; TP53; p202; p53BP1; tumor protein p53 binding protein 1
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: Double-strand break (DSB repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs in response to DNA damage by promoting non-homologous end joining (NHEJ-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub and histone H4 dimethylated at 'Lys-20' (H4K20me2, two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity. In contrast, it is not required for classic NHEJ and V(DJ recombination (By similarity. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.
Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Immunogen: Recombinant fusion protein of human TP53BP1
Dilution: IHC 1:50-1:200
Calculated MW: 213kDa/214kDa
Observed MW: Refer to figures
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