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TRAF6, GST-Tag Recombinant
TRAF6, GST-Tag Recombinant
Artikelnummer
BPS101597-1
Verpackungseinheit
2 µg
Hersteller
BPS Bioscience
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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: TRAF6 activity was measured using varying amounts of TRAF6 and incubated with E1, E2, and ATP in the presence of an "intrachain Ubiquitin" mixture for 60 minutes at 30°C in a 20-µl reaction. After incubation, TR-FRET was detected (340=>620 nm, and 340=>665 nm). The TR-FRET is proportional to the auto polyubiquitination of TRAF6.
Description: Human Recombinant TRAF6 (TNF receptor associated factor 6), encompassing amino acids 51-159. This construct contains an N-terminal GST-tag followed by a PreScission Site to facilitate removal of the GST-tag.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and variable Glutathione
Genbank: NM_145803
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-Tag
Uniprot: Q9Y4K3
Biosafety Level: Not applicable (BSL-1)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: TRAF6 activity was measured using varying amounts of TRAF6 and incubated with E1, E2, and ATP in the presence of an "intrachain Ubiquitin" mixture for 60 minutes at 30°C in a 20-µl reaction. After incubation, TR-FRET was detected (340=>620 nm, and 340=>665 nm). The TR-FRET is proportional to the auto polyubiquitination of TRAF6.
Description: Human Recombinant TRAF6 (TNF receptor associated factor 6), encompassing amino acids 51-159. This construct contains an N-terminal GST-tag followed by a PreScission Site to facilitate removal of the GST-tag.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and variable Glutathione
Genbank: NM_145803
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-Tag
Uniprot: Q9Y4K3
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101597-1 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101597-1 |
| Green Labware | Nein |
| Verpackungseinheit | 2 µg |
| Mengeneinheit | STK |
| Wirt | Escherichia Coli |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
