Application Note: Anti-TRIF antibody has been tested by WB, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 76kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunohistochemistry Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: 1:500-1:1000
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Immunogen: TRIF Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids of the mouse protein TRIF.
Physical State: Liquid
Purity and Specificity: Anti-TRIF antibody is sera. A BLAST analysis was used to suggest cross-reactivity with Anti-TRIF from mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TRIF from other sources has not been determined.
Background: Anti-TRIF antibody detects mouse TRIF. TICAM (Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule-1) or TRIF (Toll/IL-1 receptor domain-containing adapter inducing IFN-beta) plays an essential role in the MyD88-independent signaling of TLR3. Anti-TRIF antibody is ideal for investigators involved in cytokines and growth factor research.
Low Endotoxin: No