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TRPM2-HEK293 Recombinant Cell Line
TRPM2-HEK293 Recombinant Cell Line
Artikelnummer
BPS90331
Verpackungseinheit
2 vials
Hersteller
BPS Bioscience
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Applications: Monitor TRPM2 calcium channel activity
Screen for activators or inhibitors of TRPM2 calcium channel
Background: TRPM2 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRPM2 channel is a nonselective, calcium-permeablecation channel highly expressed in the brain, vascular smooth muscle, endothelial cells and immune cells. It is activated by oxidative stressors such as hydrogen peroxide, cyclic ADP ribose, and NADP, leading to an increase in the intracellular free calcium concentration and cell death. TRPM2 is a potential drug target in a variety of human diseases, including cardiovascular and neurodegenerative diseases.
Biological Activity: N-terminal FLAG tagged human TRPM2 channel has been stably integrated into HEK293 cells and its expression can be induced by tetracycline (doxycycline). The tetracycline-inducible expression of TRPM2 was confirmed by Western blotting. The function of TRPM2 was characterized by calcium assay. TRPM2-HEK293 cells were pre-treated with doxycycline to induce the expression of TRPM2, then stimulated with H2O2, and calcium influx was observed. The H2O2-induced calcium influx via TRPM2 could be blocked by N-(p-Amylcinnamoyl) anthranilic Acid (ACA), a TRP channel blocker.
Description: Stable, recombinant HEK293 cell line expressing tetracycline-inducible human TRPM2 (transient receptor potential cation channel, subfamily M, member 2, accession number NM_003307).
Genbank: NM_003307
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Tags: N-terminal FLAG tag
Uniprot: O94759
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Moran, M.M., et al. (2011). Nat. Rev. Drug Discov. 10(8):601-620
2. Song, Y., et al. (2008) J. Biomol. Screen. 13(1):54-61
3. Fonfria, E., et al. (2004). Br. J. Pharmacol. 143(1):186-192.
Applications: Monitor TRPM2 calcium channel activity
Screen for activators or inhibitors of TRPM2 calcium channel
Background: TRPM2 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRPM2 channel is a nonselective, calcium-permeablecation channel highly expressed in the brain, vascular smooth muscle, endothelial cells and immune cells. It is activated by oxidative stressors such as hydrogen peroxide, cyclic ADP ribose, and NADP, leading to an increase in the intracellular free calcium concentration and cell death. TRPM2 is a potential drug target in a variety of human diseases, including cardiovascular and neurodegenerative diseases.
Biological Activity: N-terminal FLAG tagged human TRPM2 channel has been stably integrated into HEK293 cells and its expression can be induced by tetracycline (doxycycline). The tetracycline-inducible expression of TRPM2 was confirmed by Western blotting. The function of TRPM2 was characterized by calcium assay. TRPM2-HEK293 cells were pre-treated with doxycycline to induce the expression of TRPM2, then stimulated with H2O2, and calcium influx was observed. The H2O2-induced calcium influx via TRPM2 could be blocked by N-(p-Amylcinnamoyl) anthranilic Acid (ACA), a TRP channel blocker.
Description: Stable, recombinant HEK293 cell line expressing tetracycline-inducible human TRPM2 (transient receptor potential cation channel, subfamily M, member 2, accession number NM_003307).
Genbank: NM_003307
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Tags: N-terminal FLAG tag
Uniprot: O94759
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Moran, M.M., et al. (2011). Nat. Rev. Drug Discov. 10(8):601-620
2. Song, Y., et al. (2008) J. Biomol. Screen. 13(1):54-61
3. Fonfria, E., et al. (2004). Br. J. Pharmacol. 143(1):186-192.
| Artikelnummer | BPS90331 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 90331 |
| Verpackungseinheit | 2 vials |
| Mengeneinheit | PAK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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