Tyk2, His-Tag Recombinant

Tyk2, His-Tag Recombinant
Artikelnummer
BPS40285
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience

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Encompassing Amino Acids: 871-1187(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was incubated at 30°C for 30 minutes. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Description: Human Tyk2 (Tyrosine Kinase 2, also known as JTK1), GenBank Accession No. NM_003331, a.a. 871-1187(end), with N-terminal His-tag, MW=38 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris, pH 8, 125 mM NaCl, 2.4 mM KCl, 0.045% Tween-20, 100 mM imidazole and 10% glycerol.

Genbank: NM_003331

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P29597

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Firmbach-Kraft I, et al. (1990). Oncogene 5 (9): 1329-36.
2. Partanen J, et al. (1991Proc. Natl. Acad. Sci. U.S.A. 87 (22): 8913-7.
3. Domanski P, et al. (1995). FEBS Lett. 374 (3): 317-22.
Mehr Informationen
Artikelnummer BPS40285
Hersteller BPS Bioscience
Hersteller Artikelnummer 40285
Green Labware Nein
Verpackungseinheit 10 µg
Mengeneinheit STK
Wirt Insect
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