Tyrosinase(T311), 1mg/mL

Description: Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, BSA-free

Concentration: 1 mg/mL

Storage buffer: PBS, no BSA, no azide

Product Origin: Animal - Mus musculus (mouse)

Clone: T311

Entrez Gene ID: 7299

Immunogen: Recombinant tyrosinase protein

Expected AB Applications: ELISA (published for clone)/Flow (intracellular) (published for clone)/IP (published for clone)/WB (published for clone)

Verified AB Applications: IHC (FFPE) (verified)

Z-Antibody Applications: Flow, intracellular (published)/ELISA (published)/IHC, FFPE (verified)/IP (published)/WB (published)

• Artikelnummer: BTMBNUM0012-50
• Hersteller: Biotium
• Hersteller Artikelnummer: BNUM0012-50
• Green Labware: Nein
• Verpackungseinheit: 50 uL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunoprecipitation, Western Blotting, ELISA, Flow Cytometry, Immunohistochemistry
• Isotyp: IgG2a kappa
• Wirt: Mouse