UBA6 (UBE1L2), FLAG-tag Recombinant

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Encompassing Amino Acids: 2-end

Applications: Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay Conditions: Reaction mixturecontaining UBA6 (UBE1L2) as a positivecontrol, Ubc5a (500 ng), and FLAG-Smurf2 (500 ng) was incubated at 37°Cfor 30 minutes with biotinylated ubiquitin. The reaction mix was incubated withAnti-FLAG for 1 hour, then HRP secondaryAb for 30 min, followed bychemiluminescent detection.

Description: Human UBA6, also known as UBE1L2 (GenBank Accession No. NM_018227), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 80 µg/mlFLAG peptide, 20% glycerol, and 3 mM DTT.

Genbank: NM_018227

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag

Uniprot: A0AVT1

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Groettrup M. et al., Trends Biochem. Sci. 33 (5), 230-237 (2008).
2. Pelzer C. et al., J. Biol. Chem. 282 (32), 23010-23014 (2007).

• Artikelnummer: BPS80303
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80303
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK