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Encompassing Amino Acids: 2-end
Applications: Useful in conjunction with E1 and E3 for the studies of enzyme kinetics, screening inhibitors and selectivity profiling.
Description: Human UbcH5c, also known as UBE2D3, (GenBank Accession No. NM_001300795), amino-acids 2-end, with N-terminal His tag, MW=18 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 220mM Imidazole, 0.04% Tween20, 20% glycerol
Genbank: U39318.1
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal His-tag
Uniprot: P61077
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Markson G. et al., Genome Res. 19, 1905-1911 (2009).
2. Nuber U. and Scheffner M., J. Biol. Chem. 274 (11), 7576-7582 (1999).