UBCH5c TR-FRET Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for screening small molecular inhibitors for drug discovery and HTS applications.

Description: UBCH5c (UBE2D3) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5c TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5c protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

Storage Stability: At least 6 months at -80°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Saville, M.K., et al., J. Biol. Chem. 2004; 279(40): 42169-81.
2. Kim, J.-H., et al., BMB Rep. 2015; 48(1): 25-29.

• Artikelnummer: BPS79901
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79901
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK