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UbcH6 (UBE2E1), His-tag Recombinant
UbcH6 (UBE2E1), His-tag Recombinant
Artikelnummer
BPS80316
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-end
Applications: Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 as an E1 (0.5 µg), UbcH6, Biotin-Ubiquitin (0.5 µg) and Smurf2 as an E3(2 µg) in 50 mM Tris pH 7.4) for 30 min at 37°C, then add to streptavidin coated plates for 30 min. at 37°C. Incubate with anti-Smurf2, thenEuropium-labeled secondary antibody, and detect by time resolved fluorescence (λex=330 nm, λem=620 nm).
Description: UbcH6, also known as UBE2E1 (GenBank Accession No. NM_003341), a.a. 2-end, with N-terminal His-tag, MW = 22 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_003341
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P51965
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sommer, T., et al. 1993. Nature 365 (6442):176-9.
2. Yang, M. et al. 1997. J. Biol. Chem. 272 :1970-1975.
Encompassing Amino Acids: 2-end
Applications: Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 as an E1 (0.5 µg), UbcH6, Biotin-Ubiquitin (0.5 µg) and Smurf2 as an E3(2 µg) in 50 mM Tris pH 7.4) for 30 min at 37°C, then add to streptavidin coated plates for 30 min. at 37°C. Incubate with anti-Smurf2, thenEuropium-labeled secondary antibody, and detect by time resolved fluorescence (λex=330 nm, λem=620 nm).
Description: UbcH6, also known as UBE2E1 (GenBank Accession No. NM_003341), a.a. 2-end, with N-terminal His-tag, MW = 22 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_003341
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P51965
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sommer, T., et al. 1993. Nature 365 (6442):176-9.
2. Yang, M. et al. 1997. J. Biol. Chem. 272 :1970-1975.
| Artikelnummer | BPS80316 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80316 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
