UBE2A, His-Tag Recombinant

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Applications: Useful for the study of ubiquitin enzyme kinetics, and as a positive control for Western blot studies.

Background: UBE2A or ubiquitin-conjugating enzyme E2A is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. RAD18 and RAD5 play a central role in mediating physical contacts between the members of the RAD6 pathway. UBE2A is specifically important during chronic low dose ultraviolet exposure to prevent counterproductive DNA checkpoint activation and allow cells to proliferate normally. Loss of the RAD6 error-free PRR pathway results in DNA damage checkpoint-induced G2 arrest in CLUV-exposed cells.

Description: Recombinant full-length Human UBE2A, also known as HHR6A, MRXS30, MRXSN, RAD6A, and UBC. Genbank Accession No. NM_003336, with an N-terminal His-tag, expressed in E. coli. MW=17 kDa.

Format: Aqueous buffer solution

Formulation: 50 mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, and 25% glycerol.

Genbank: NM_003336

Storage Stability: At least 6 months at -70⁰C. Avoid freeze/thaw cycles.

Tags: N-Terminal His-Tag

Uniprot: P49459

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Mondal, S., et al., Anal. Biochem. 2016; 510: 41-51.2. Hishida, T., et al., Nature. 2009; 457: 612-615.

• Artikelnummer: BPS79368
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79368
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK