UBE2C, His-Tag Recombinant

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Applications: Useful for the study of ubiquitin enzyme kinetics, and as a positive control for Western blot studies.

Background: UBE2C or ubiquitin-conjugating enzyme E2C is a member of the E2 ubiquitin-conjugating enzyme family which is required for the destruction of mitotic cyclins and for cell cycle progression. UBE2C acted as a dominant-negative inhibitor which blocks the destruction of mitotic cyclins A and B and the onset of anaphase in frog embryos and mammalian cells. High UBE2C mRNA expression is associated with poor disease-free survival and overall survival. High tumor grade, as well as high Ki67 protein expression, was more frequent in the high-expression group of UBE2C.

Description: Recombinant full-length Human UBE2C, also known as UBCH10. Genbank Accession No. NM_007019, with an N-terminal His-tag, expressed in E. coli. MW=21 kDa.

Format: Aqueous buffer solution

Formulation: 50 mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, and 25% glycerol.

Genbank: NM_007019

Storage Stability: At least 6 months at -70⁰C. Avoid freeze/thaw cycles.

Tags: N-Terminal His-Tag

Uniprot: O00762

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Mondal, S., et al., Anal. Biochem. 2016; 510: 41-51.2. Rape, M., et al., Nature. 2004; 432: 588-595.

• Artikelnummer: BPS79369
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79369
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK