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Ubiquitin AMC
Ubiquitin AMC
Artikelnummer
BPS81150
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Amino Acid Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Applications: Useful as a substrate for deubiquitinating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs), as well as investigation of deconjugating enzyme substrate specificity and for screening for modulators of
Assay Conditions: Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Background: Ubiquitin-AMC is prepared via the conjugation of the C-terminus of mature, Ubiquitin with 7-amino-4-methylcoumarin (AMC). This conjugation quenches the intrinsic fluorescence of AMC. The high purity of BPS's Ubiquitin-AMC compared to that of other commercial vendors leads to a greater signal:background.
Biological Activity: Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM.
Description: Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags.
Format: Lyophilized solid
Genbank: P62987 (ubiquitin)
Purity: ≥95% by HPLC.
Solubility: Soluble in DMSO or aqueous buffers (> 1 mg/ml)
Storage Stability: Store in the dark at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -80°C. Store DMSO solutions at -80°C for up to 1 month. Avoid freeze/thaw cycles of solutions.
Tags:
Uniprot: P09936
Warnings: Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dang, L.C., et al. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry, 37, 1868-1879 (1998).
2. Mason, D.E., et al. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry. Biochemistry, 43, 6535-44 (2004).
Amino Acid Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Applications: Useful as a substrate for deubiquitinating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs), as well as investigation of deconjugating enzyme substrate specificity and for screening for modulators of
Assay Conditions: Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Background: Ubiquitin-AMC is prepared via the conjugation of the C-terminus of mature, Ubiquitin with 7-amino-4-methylcoumarin (AMC). This conjugation quenches the intrinsic fluorescence of AMC. The high purity of BPS's Ubiquitin-AMC compared to that of other commercial vendors leads to a greater signal:background.
Biological Activity: Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM.
Description: Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags.
Format: Lyophilized solid
Genbank: P62987 (ubiquitin)
Purity: ≥95% by HPLC.
Solubility: Soluble in DMSO or aqueous buffers (> 1 mg/ml)
Storage Stability: Store in the dark at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -80°C. Store DMSO solutions at -80°C for up to 1 month. Avoid freeze/thaw cycles of solutions.
Tags:
Uniprot: P09936
Warnings: Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dang, L.C., et al. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry, 37, 1868-1879 (1998).
2. Mason, D.E., et al. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry. Biochemistry, 43, 6535-44 (2004).
| Artikelnummer | BPS81150 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 81150 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
