USP2, FLAG-Tag (Sf9-derived) Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-605(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP2 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal wasmeasured at excitation =340 nm, emission =460 nm.

Description: Human USP2 (Ubiquitin-Specific Protease 2) or UPB41, GenBank Accession No. NM_004205, a.a. 2-605(end) with an N-terminal FLAG-tag, MW=68 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl Flag peptide, 20% glycerol, and 3 mM DTT.

Genbank: NM_004205

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag

Uniprot: O75604

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Stevenson, L.F., et al. (2010). EMBO J. 140(3): 384-96.
2. Priolo, C., et al. (2006). Cancer Res. 66(17): 8625-32.
3. Haimerl, F., et al. (2009). J. Biol. Chem. 284(1): 495-504.

• Artikelnummer: BPS80352
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80352
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK