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USP8, FLAG-tag Recombinant
USP8, FLAG-tag Recombinant
Artikelnummer
BPS80358
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-1118(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP8 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
Description: Human USP8 (Ubiquitin-Specific Protease 8) or UBPY, GenBank Accession No. NM_005154, a.a. 2-1118(end) with an N-terminal FLAG-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Genbank: NM_005154
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P40818
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Naviglio, S., et al. (1998). EMBO J. 17(12):3241-50.
2. Niendorf, S., et al. (2007). Mol Cell Biol. 727(13):5029-39.
3. Mizuno, E., et al. (2005). Mol. Biol. Cell. 16(11): 5163-74.
Encompassing Amino Acids: 2-1118(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP8 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
Description: Human USP8 (Ubiquitin-Specific Protease 8) or UBPY, GenBank Accession No. NM_005154, a.a. 2-1118(end) with an N-terminal FLAG-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Genbank: NM_005154
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P40818
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Naviglio, S., et al. (1998). EMBO J. 17(12):3241-50.
2. Niendorf, S., et al. (2007). Mol Cell Biol. 727(13):5029-39.
3. Mizuno, E., et al. (2005). Mol. Biol. Cell. 16(11): 5163-74.
| Artikelnummer | BPS80358 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80358 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
