UTX (KDM6A), N-terminal FLAG-tag Recombinant

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Encompassing Amino Acids: 1-1401

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 0.5 µM of 3MeK27 H3 21-44[Biotin] is incubated for 1 hr incubation at RT with varying concentrations of UTX in 1x UTX Assay Buffer, then 5 uL of anti rabbit acceptor beads (1:250 dilution) + 5 uL of primary ab 6 (1:125 dilution) is added and incubated at RT for 30 min followed by 10 µL of streptavidin donor beads (1:125) for 30 min and alpha counts are read.

Description: Human UTX, also known as KDM6A (GenBank Accession No. NM_021140) (amino-acids 1-1401) with N-terminal FLAG-tag, MW = 155 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 80 µg/ml FLAG peptide

Genbank: NM_021140

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary.

Tags: N-terminal FLAG-tag

Uniprot: O15550

Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Greenfield A, et al. (1998). Hum. Mol. Genet. 7 (4): 737-42.
2. Agger K, et al. (2007). Nature 449 (7163): 731-4.Application Reference(s):
Identification of the KDM2/7 histone lysine demethylase subfamily inhibitor and its antiproliferative activity (2013)

• Artikelnummer: BPS50119
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50119
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect