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Encompassing Amino Acids: 27-191
Application: Useful for cell culture and for the study of signaling and developmental pathways.
Assay Conditions: VEGF165 was tested using VEGFR2 / NFAT Reporter – HEK293 Recombinant Cell Line (BPS Bioscience #79387).
Biological Activity: ED50 is less than 3 ng/ml as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
Description: Human VEGF165, also known as Vascular endothelial growth factor 165, and VEGF, a.a. 27-191 with N-terminal His-Tag. GenBank Accession number NM_001025368, and expressed in a Baculovirus infected Sf9 cell expression system. MW = 20 kDa.
Endotoxin Level: < 1EU per µg of VEGF as determined by the LAL method.
Format: lyophilized protein
Genbank: NM_001025368
Purity: ≥90%
Reconstitution: Reconstitute in sterile dH2O.
Storage Stability: At least 12 months at –80°C (as a solid). Store reconstituted protein in aliquots at -80°C for up to 6 months.
Tags: N-terminal His-Tag
Uniprot: P15692
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ruckman, J., et al. Journal of Biological Chemistry. 273, 20556-20567 (1998).2. Soker, S., et al. Cellular Biochemistry. 85(2), 357-368 (2002).
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