VGLUT1 Antibody, Clone N28/9: ATTO 390

Mouse Anti-Rat VGLUT1 Monoclonal IgG1

Target: VGLUT1

Conjugate: ATTO 390

Product Type: Monoclonal

Clone Number: N28/9 (Formerly sold as S28-9)

Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Swiss-Prot: Q62634

Purification: Protein G Purified

Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

Concentration: 1 mg/ml

Specificity: Detects ~52kDa. No cross-reactivity against VGlut2.

Cellular Localization: Cytoplasmic Vesicle,Secretory Vesicle,Synaptic Vesicle Membrane,Membrane,Cell Junction,Synapse

Scientific Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

References: 1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163.2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSMC-394D-A390
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SMC-394D-A390
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus)
• Klonalität: Monoclonal
• Methode: Western Blotting, Immunohistochemistry
• Isotyp: IgG1
• Human Gene ID: 116638
• Wirt: Mouse