VHL/CUL2/ELOB/ELOC/RBX1 Complex Recombinant

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Applications: Useful for the study of protein degradation and PROTAC design, and selectivity profiling.

Assay Conditions: ARV-771-mediated binding of VHL BPS #100373 to BET bromodomain was assessed using A-screen technology. Various amounts of VHL were incubated in 10-ul reaction with ARV-771 and GSTBRD3(BD2) BPS #31033 at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.

Description: VHL/CUL2/ELOB/ELOC/RBX1 complex of human VHL (GenBank Accession No. NM_000551) a.a. 2-212(end) with N-terminal FLAG-tag and MW= 25 kDa, human CUL2 (GenBank Accession No. NM_003591), a.a. 2-745(end) with N-terminal FLAG-tag and MW= 88 kDa, human ELOB (GenBank Accession No. NM_007108), a.a. 2-118 with N-terminal FLAG-tag and MW= 14 kDa, ELOC (GenBank Accession No. NM_005648), a.a. 2-112(end) with N-terminal His-tag and MW= 13 kDa human Rbx1 (GenBank Accession No. NM_014248), a.a. 2-108(end) with N-terminal His-tag and MW= 13 kDa, individually expressed in a HEK293 expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT.

Genbank: VHL: NM_000551; CUL2: NM_003591; ELOB: NM_007108; ELOC: NM_005648; Rbx1: NM_005648

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: VHL: N-terminal FLAG-tag; CUL2: N-terminal FLAG-tag; ELOB: N-terminal FLAG-tag; ELOC: N-terminal His-tag; Rbx1: N-terminal His-tag

Uniprot: VHL: P40337; CUL2: Q13617; ELOB: Q15370; ELOC: Q15369; Rbx1: P62877

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Kamura, T., et al., Science. 1999; 284(5414): 657-661.
2. Stebbins, C.E., et al., Science. 1999; 284(5413): 455-461.

• Artikelnummer: BPS100373-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100373-2
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK