WNK3, GST-Tag Recombinant

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Encompassing Amino Acids: 1-571

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µl of varying amounts of WNK3 was mixed with 10 µl of 0.5 mg/ml MBP protein substrate. Reaction was initiated by adding 5 µl of 250 µM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.

Background: WNK3 is a member of serine-threonine protein kinases, which was named the with-no-lysine family. WNK3 is expressed in several tissues including kidney and brain. WNK3 can regulate the activity of the electroneutral cation-coupled chloride cotransporters by activating NKCC1/2 and NCC and inhibiting KCCs. WNK3 kinase function is required for normal blood pressure homeostasis and the control of neuronal excitability. Also, overexpression of WNK3 increases the survival of Hela cells through caspase-3 pathway.

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_001002838

Purity: 0,9

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal GST-tag

Uniprot: Q9BYP7

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS100973
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100973
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK