English
XIAP, FLAG-Tag Recombinant
XIAP, FLAG-Tag Recombinant
Artikelnummer
BPS80401
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-end
Applications: Useful as an Ubiquitin ligase in conjunction with E1 and E2 for the study of enzyme kinetics. Ideal for Bir Domain binding and inhibition studies of Caspases 3 7, and 9. Also useful for screening inhibitors, and selectivity profiling.
Assay Conditions: Ligase Assay Conditions:
50 µl of reaction mix (4 mM ATP, 5mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2(2.8 µg), Biotin-Ubiquitin (0.5 µg) andXIAP (0-1.25 µg) in 50 mM Tris pH 7.4)is incubated for 30 min at 37°C.Transfer the reaction mixture to astreptavidin-coated plate and incubatefor 30 min at 37°C. Add antibody againstXIAP, incubate 1 hr. Then, addsecondary HRP-labeled antibody andincubate 30 min. Finally, add HRPchemiluminescent substrates andread luminescence.
Bir Domain Assay Conditions:
50 mM Tris-HCl, pH 7.4, 25 mM NaCl,0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutesat room temperature. Fluorescencepolarization is measured at 475 nm/528 nmof exc/em wavelength.
Description: Human X-linked inhibitor of apoptosis (XIAP), also known as IAP-like protein, API3, X-linked IAP, and BIRC4 (GenBank Accession No. NM_001167), a.a. 2-end, with N-terminal FLAG-tag, MW= 57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
Genbank: NM_001167
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P98170
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gu L. et al., Cancer Cell 15 (5), 363-375 (2009).
2. Deveraux Q.L. et al., Nature 388 (6639), 300-304 (1997).
Encompassing Amino Acids: 2-end
Applications: Useful as an Ubiquitin ligase in conjunction with E1 and E2 for the study of enzyme kinetics. Ideal for Bir Domain binding and inhibition studies of Caspases 3 7, and 9. Also useful for screening inhibitors, and selectivity profiling.
Assay Conditions: Ligase Assay Conditions:
50 µl of reaction mix (4 mM ATP, 5mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2(2.8 µg), Biotin-Ubiquitin (0.5 µg) andXIAP (0-1.25 µg) in 50 mM Tris pH 7.4)is incubated for 30 min at 37°C.Transfer the reaction mixture to astreptavidin-coated plate and incubatefor 30 min at 37°C. Add antibody againstXIAP, incubate 1 hr. Then, addsecondary HRP-labeled antibody andincubate 30 min. Finally, add HRPchemiluminescent substrates andread luminescence.
Bir Domain Assay Conditions:
50 mM Tris-HCl, pH 7.4, 25 mM NaCl,0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutesat room temperature. Fluorescencepolarization is measured at 475 nm/528 nmof exc/em wavelength.
Description: Human X-linked inhibitor of apoptosis (XIAP), also known as IAP-like protein, API3, X-linked IAP, and BIRC4 (GenBank Accession No. NM_001167), a.a. 2-end, with N-terminal FLAG-tag, MW= 57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
Genbank: NM_001167
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P98170
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gu L. et al., Cancer Cell 15 (5), 363-375 (2009).
2. Deveraux Q.L. et al., Nature 388 (6639), 300-304 (1997).
| Artikelnummer | BPS80401 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80401 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
