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Yes1, GST-tag Recombinant
Yes1, GST-tag Recombinant
Artikelnummer
BPS40488
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done inKinase buffer containing 0.2 mM DTTusing Poly-(Glu4:Tyr)-biotin substrate(0.2 mg/ml) and 20 µM ATP. Reactionwas done at 30°C for 45 min. Amount ofATP transferred was calculated usingKinase-Glo reagent (Promega)
Background: YES1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene that has tyrosine kinase activity and belongs to the SRC family. YES1 lies in close proximity to thymidylate synthase gene on chromosome 18 and chromosome 22 (1). The activation of YES1 may play a significant role in the malignant transformation of hepatocytes, and is important for maintaining embryonic stem cells in an undifferentiated state. YES1 is a useful marker to detect early-stage hepatocellular carcinoma, and it plays a key role in the tumorigenesis and metastasis of gastric cancer. YES1 induction results in increased cancer cell motility suggesting that YES1 may promote cancer spread and metastasis rather than tumor growth (2).
Description: Human, full length, YES1, also known as Yes, c-yes, HsT441, P61-YES GenBank Accession No. NM_005433, with N-terminal GST tag, a.a. 2- 543(end), MW 88 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3mM DTT, and 20% glycerol.
Genbank: NM_005433
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P07947
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Silverman, G. et.al, Genomics 15: 442-445, 1993.
2. Barraclough, J. et al, Neoplasia. 2007 Sep;9(9):745-54.
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done inKinase buffer containing 0.2 mM DTTusing Poly-(Glu4:Tyr)-biotin substrate(0.2 mg/ml) and 20 µM ATP. Reactionwas done at 30°C for 45 min. Amount ofATP transferred was calculated usingKinase-Glo reagent (Promega)
Background: YES1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene that has tyrosine kinase activity and belongs to the SRC family. YES1 lies in close proximity to thymidylate synthase gene on chromosome 18 and chromosome 22 (1). The activation of YES1 may play a significant role in the malignant transformation of hepatocytes, and is important for maintaining embryonic stem cells in an undifferentiated state. YES1 is a useful marker to detect early-stage hepatocellular carcinoma, and it plays a key role in the tumorigenesis and metastasis of gastric cancer. YES1 induction results in increased cancer cell motility suggesting that YES1 may promote cancer spread and metastasis rather than tumor growth (2).
Description: Human, full length, YES1, also known as Yes, c-yes, HsT441, P61-YES GenBank Accession No. NM_005433, with N-terminal GST tag, a.a. 2- 543(end), MW 88 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3mM DTT, and 20% glycerol.
Genbank: NM_005433
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P07947
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Silverman, G. et.al, Genomics 15: 442-445, 1993.
2. Barraclough, J. et al, Neoplasia. 2007 Sep;9(9):745-54.
| Artikelnummer | BPS40488 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40488 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
