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Encompassing Amino Acids: 33-501
Applications: Useful for protein binding studies, screening inhibitors of proteinprotein interactions, and studying protein function.
Description: Zaire Ebola Virus (Zebov)glycoprotein 1 (GP1), also known asenvelope glycoprotein with aC-terminal His-tag, GenBank AccessionNo. NP_066246, MW=57.1 kDa,expressed in a HEK293 cell expressionsystem. This protein runs at an apparent higher molecular weight due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NP_066246
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Lee, J.E., et al., Nature. 2008; 454(7201): 177-182.
2. Dube, D., et al., J. Virol. 2009; 83(7): 2883- 2891.