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Application: Luciferase- based analysis of live and dead target cells in cytotoxicity assays.Test the efficacy of multi-specific immune engager molecules.Assess the Fc effector function of candidate antibodies.
Background: Lymphocyte-mediated cytotoxicity is a form of cellular immunity against intracellular pathogens, including viruses and certain bacteria and parasites. The most popular in vitro methods to monitor lymphocyte-mediated cytotoxicity on target cells are cell-mediated cytotoxicity assays such as ADCC (antibody-dependent cellular cytotoxicity) and TDCC (T-cell dependent cellular cytotoxicity) in which immune effector cells and target cells are co-cultured. To analyze immune effector cell cytolytic activity in such heterogeneous cell population of effector and target cells, it is important to be able to discriminate between effector and target cell populations with distinct phenotypes. The use of luciferase allows for a clear separation between the effector and the surviving target cells. The instability of firefly luciferase when released from dead target cells in cell culture gives it a half-life of approximately 2 hours, eliminating any residual luminescence signal generated from dead target cells. Cytotoxicity assays are crucial to understand the potency of CAR (chimeric antigen receptor) T and NK cells, and antibody-based immunotherapies. The Raji line was established from a Burkitt's lymphoma patient. Raji cells constitutively express B cell antigens CD19, CD20, and CD22, and offer a physiologically relevant platform to evaluate cancer-directed immunotherapies such as Chimeric Antigen Receptor (CAR) T-cells. Firefly Luciferase Raji Cell Line is a Raji cell line constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter. These are suspension cells.
Description: The PBMC Cytotoxicity Luciferase Assay Kit (Raji) is a kit designed to determine the cytotoxicity profile of PBMCs (Peripheral Blood Mononuclear Cells) towards the Firefly Luciferase Raji Cell Line. It uses the luminescence signal from the Firefly Luciferase Raji Cell Line to measure the number of live target cells within a mixed cell population of PBMC and Firefly Luciferase Raji cells. The kit contains PBMCs, Firefly Luciferase Raji Cell Line, cell culture media, recombinant human interleukin-2 for making assay medium, and One-Step™ Luciferase Assay System. In addition, this kit includes a positive control antibody.Purchase of the Raji cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions at https://bpsbioscience.com/license
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: This product is for research purposes only and not for human or therapeutic use. This product should be considered hazardous and is harmful by inhalation, in contact with skin, eyes, clothing, and if swallowed. If contact occurs, wash thoroughly.
Biosafety Level: BSL-1
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