NK Cell Expansion Kit 2

Products from BPS Bioscience require a minimum order value above 400€

Application: Culture and expansion of primary NK cells.

Background: NK (natural killer) cells are part of the innate immune system. They function in a histocompatibility complex-independent mode and derive from the hematopoietic lineage. They are the first line of defense against cancer. Expression of marker CD56 correlates with NK cell functionality: the CD56bright subset accounts for about 5% of the population and is less cytotoxic than the CD56dim subset.  Cytotoxicity can happen by the release of perforin and granzyme, while activation by KARs (killer activating receptors) leads to release of Fas Ligand, TRAIL (TNF-related apoptosis-inducing ligand) and TNFα (tumor necrosis factor-alpha). In a suppressive tumor microenvironment, NK cells can become inhibited and unable to fight cancer cells. Several clinical trials have focused on using ex vivo generated NK cells alone or in combination with other approaches. NK cells can be generated ex vivo from peripheral blood, umbilical cord blood, iPS cells or immortalized NK cell lines. The ability to generate a number of pure cells high enough for human dosage often requires the use of growth factors such as IL-2 (interleukin 2) or IL-15, and feeder cells. A recent study indicates that the sequential use of mbIL-21-based followed by mbIL-15-based feeder cells during NK expansion optimizes the yield and function of primary NK cells, this may be the optimal approach in many NK expansion, engineering, and functional study settings. The use of NK cells or CAR (chimeric antigen receptor)-NK cells is an expanding area holding great promise in cancer therapy. 

Description: The NK Cell Expansion Kit 2 is suitable for the ex vivo culture and expansion of human natural killer (NK) cells. It contains engineered Membrane Bound IL-15 Growth-Arrested NK Feeder Cells and NK Medium, Serum-Free. Membrane Bound IL-15 Based Growth-Arrested NK Feeder Cells are K562 cells engineered to express membrane bound IL-15, and other components, to drive superior functionality of expanded NK cells. The kit provides enough growth-arrested feeder cells for a starter population of 1 million purified NK cells or PBMC cells.

Format: Aqueous buffer solution

Storage Stability: At least 6 months at -80°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

References: Du N., et al., 2021 Cancers (Basel) 13 (16): 4129.Zhang C., et al., 2023 Cancer Immunol Res 11 (11): 1524-1537.

• Artikelnummer: BPS82393
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82393
• Verpackungseinheit: 1 kit
• Mengeneinheit: PAK