PBMC Cytotoxicity Luciferase Assay Kit (Ramos)

Products from BPS Bioscience require a minimum order value above 400€

Application: Luciferase- based analysis of live and dead target cells in cytotoxicity assays.Test the efficacy of multi-specific immune engager molecules.Assess the Fc effector function of candidate antibodies.

Background: Lymphocyte-mediated cytotoxicity is a form of cellular immunity against intracellular pathogens, including viruses and certain bacteria and parasites. The most popular in vitro methods to monitor lymphocyte-mediated cytotoxicity on target cells are cell-mediated cytotoxicity assays such as ADCC (antibody-dependent cellular cytotoxicity) and TDCC (T-cell dependent cellular cytotoxicity) in which immune effector cells and target cells are co-cultured. To analyze immune effector cell cytolytic activity in such heterogeneous cell population of effector and target cells, it is important to be able to discriminate between effector and target cell populations with distinct phenotypes. The use of luciferase allows for a clear separation between the effector and the surviving target cells. The instability of firefly luciferase when released from dead cells in cell culture gives it a half-life of approximately 2 hours, minimizing any residual luminescence signal generated from dead target cells. Cytotoxicity assays are crucial to understand the potency of CAR (chimeric antigen receptor) T and NK cells, and antibody-based immunotherapies.Ramos is a human Burkitt's lymphoma cell line grown in suspension. The eGFP/Firefly Luciferase Ramos cell line is a Ramos cell line expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. Luciferase activity is directly proportional to live cell numbers.

Description: The PBMC Cytotoxicity Luciferase Assay Kit (Ramos) is designed to determine the cytotoxicity profile of PBMCs (Peripheral Blood Mononuclear Cells) towards the eGFP/Firefly Luciferase Ramos (RA 1) cell line. It uses the luminescent signal from the eGFP/Firefly Luciferase Ramos cell line to measure the number of live target cells within a mixed cell population of PBMC and eGFP/Firefly Luciferase Ramos cells. The kit contains PBMCs, the eGFP/Firefly Luciferase Ramos cell line, cell culture media and ONE-Step™ Luciferase Assay System. In addition, this kit includes a positive control bispecific antibody.Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Host Cell Line: Ramos (RA 1)

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

• Artikelnummer: BPS82694
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82694
• Verpackungseinheit: 1 kit
• Mengeneinheit: PAK
• Wirt: Human