Vγ4Vδ1 TCR NFAT-Luciferase Reporter Jurkat Cell Line

Products from BPS Bioscience require a minimum order value above 400€

Application: Screen Vγ4Vδ1 TCR agonist antibodies.Positive control for Vγ4Vδ1 TCR evaluation and optimization of experimental conditions.

Background: γδ TCRs (T cell receptors), αβ TCRs, and antibodies, result from gene rearrangements and offer the immune system the possibility to recognize several different types of antigens. γδ TCRs recognize antigens in a similar way to antibodies, being able to recognize full protein antigens and being independent on antigen binding to the MHC (major histocompatibility complex). γδ TCRs are cell type-specific, with Vγ4Vδ1 being present in γδTIL (tumor infiltrating lymphocytes) cells. Vδ1 expressing cells are found in mucosal and epithelial tissues and correspond to about 15% of the γδT cells present in PBMCs, with the % of γδ being only 5% of all the T cells. Vγ4Vδ1 cells can be activated by CD1 (cluster of differentiation 1) and respond to BTNL3 (butyrophilin-like 3), BTNL8, annexin A2 and A6. These cells can lead to tumor cell death by lysis. While most of the studies have been focusing on Vγ9Vδ2 T cells, a better understanding of the function and therapeutic potential of Vγ4Vδ1 T cells may open new avenues in cancer therapy.

Description: The Vγ4Vδ1 TCR NFAT-Luciferase Reporter Jurkat Cell Line was generated from the T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (#78556) by overexpression of human Vγ4Vδ1 TCR using lentiviral transduction (with Vγ4Vδ1 TCR Lentivirus #78986). This cell line was functionally validated with a TCR Vδ1 agonist antibody.

Host Cell Line: Jurkat (clone E6-1), human T lymphoblast, suspension

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

References: Allison T. and Garboczi D., 2002 Molecular Immunology 38 (14): 1051-1061.
Song Y., et al., 2022 Front Immunol 13: 914839.

• Artikelnummer: BPS82329
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82329
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK