TDO Cellular Activity QuickDetect™ Supplements

Products from BPS Bioscience require a minimum order value above 400€

Applications: • Monitor TDO pathway activity • Screen for activators or inhibitors of TDO in a cellular context

Background: L-Trp is an essential amino acid necessary for protein synthesis in mammaliancells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate andadaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressivemicroenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trpthat have immunosuppressive functions. TDO is upregulated in many tumors, providingcancer cells with an avenue for immune evasion.

Description: The TDO Cellular Activity QuickDetectTM Supplements are designed tocomplement the hTDO-HEK293 Recombinant Cell Line (BPS Cat. #60534) or other TDO-expressingcell lines. This kit contains the cell culture medium supplements necessary foractivation of TDO and for the analysis and detection of tryptophan 2,3 dioxygenase (TDO)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and thedetection reagents, when used as described, allow for indirect measurement of Kyn levels byanalyzing absorption at 480 nm.

Storage Stability: Stable for at least 6 months when stored as directed.

Warnings: *Note: both trichloroacetic acid and acetic acid are strongly corrosive acids; please use gloves and appropriate protective clothing.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS62002-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 62002-1
• Green Labware: Nein
• Verpackungseinheit: 100 rxns.
• Mengeneinheit: PAK