ProCHO 5 Protein-free CHO Medium is developed specifically to facilitate the production and downstream processing of recombinant proteins expressed in CHO cells. These protein-free formulations support high-density cultures without the need for animal derived components. Very low levels of human recombinant insulin facilitate both downstream purification and regulatory compliance. ProCHO 5 Protein-free CHO Medium for CHO cells already growing in suspension. Supports increased protein production. Contains 0.1% Pluronic® F-68. Does not contain L-glutamine, phenol red, hypoxanthine, or thymidine. ProCHO 5 Protein-free CHO Medium can be used in a fed-batch cell culture by adding feeds for example eCHO Feed, power feed A and CHO Xtreme Feed.