PowerCHO Media are hydrolysate-free, serum-free, and non-animal origin media for supporting high-density CHO cells in suspension. For therapeutic bioprocessing applications, these protein-free formulations also facilitate both downstream purification and regulatory compliance. PowerCHO contains HEPES and Pluronic® F-68, but does not contain L-glutamine, phenol red, hypoxanthine, and thymidine. PowerCHO 2 Chemically Defined, Serum-free CHO Media is optimized for both cell growth and protein production. The 10 L bag provides a flexible single use approach.