Anti-Mesothelin CAR-T Cells

Products from BPS Bioscience require a minimum order value above 400€

Applications: Use as positive control for anti-Mesothelin CAR-T cellsScreen modulators of anti-Mesothelin CAR-T cytotoxicityDesign and optimize co-culture cytotoxicity assays

Background: Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently overexpressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy approaches, including drug-conjugated antibodies and chimeric antigen receptor T cells (CAR-T Cells).

Description: The Anti-mesothelin CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-Mesothelin CAR Lentivirus (Mesothelin ScFv-CD8-4-1BB-CD3ζ; BPS Bioscience #78703). These ready-to use CAR-T cells express an anti-Mesothelin CAR consisting of the scFv (Single chain fragment variable) of anti-Mesothelin (clone P4) linked to a 2^nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.Figure 1: Construct diagram showing components of the anti-Mesothelin CAR expressed in anti-Mesothelin CAR-T cells.

Mycoplasma Testing: The cells have been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. 

Supplied As: Each vial contains 2 x 10⁶ cells in 1 ml of CryoStor® CS10 (Stemcell Technologies)

Uniprot: Q13421

Warnings: Avoid freeze/thaw cycles.Donors have been screened and determined negative for:Hepatitis B (anti-HBc EIA, HBsAg EIA)Hepatitis C (anti-HCV EIA)Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)Human T-Lymphotropic Virus (HTLV-I/II)HIV-1/HCV/HBVWest Nile VirusTrypanasoma cruziNote: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 precautions should be used.

Biosafety Level: BSL-1

• Artikelnummer: BPS78729
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78729
• Verpackungseinheit: 1 vial
• Mengeneinheit: STK
• Wirt: Human