CD33- CHO K1 Recombinant Cell Line (High, Medium and Low Expression)-79935-H

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Amino Acid Sequence: Human CD33/Siglec-3 (accession number: BC028152.1) was cloned into pCMV3.

Application: Screen for activators or inhibitors of antibody-mediated signaling for immunotherapy research and drug discovery.
Characterize CD33 antibodies and ligands for binding assay.

Background: CD33 or Siglec-3 is a transmembrane receptor expressed on cells of myeloid lineage that belongs to the sialic acid-binding immunoglobulin (Ig)-like family. CD33 can be stimulated by sialic acid residues on glycoproteins or glycolipids, resulting in a signaling cascade that inhibits phagocytosis in cells and suppresses immune cell proliferation. CD33 overexpression is one of top-ranked Alzheimer's disease risk factors and CD33 is the target of gemtuzumab ozogamicin, an antibody-drug for the treatment of patients with acute myeloid leukemia.

Description: Recombinant CD33 -CHO K1 cell line stably expressing the full length human C33/Siglec-3 receptor (GenBank Accession: BC028152.1). Surface expression of CD33 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD33 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD33 expression levels.

Genbank: BC028152.1

Host Cell Line: CHO-K1

Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza,#LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.

Storage Stability: Store in liquid nitrogen immediately upon receipt.

Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• Artikelnummer: BPS79935-H
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79935-H
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Hamster