eGFP/Firefly Luciferase OVCAR3 Cell Line

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Applications: • In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging.• Use as target cells in CAR-T co-culture killing assays

Background: The OVCAR3 cell line was isolated from a high-grade serous ovarian adenocarcinoma patient refractory to treatment with cisplatin. This cell line is highly tumorigenic and presents an abnormal karyotype. OVCAR3 cells are a suitable model of ovarian cancer for drug resistance studies and hormonal therapy.  The presence of eGFP and luciferase allow for easy assay readouts, making this cell line a convenient choice.

Description: eGFP/Firefly Luciferase OVCAR3 Cell Line is the human ovarian cancer cell line OVCAR3 engineered to express firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. This cell line was generated by transduction with Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #78741).

Host Cell Line: OVCAR3

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

• Artikelnummer: BPS78953
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78953
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human