GITR / NF-κB Luciferase Reporter - Jurkat Cell Line

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Applications: This cell line is suitable for monitoring the activity of NF-kB signaling in response to GITR ligand (GITRL).

Background: Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies.

Description: This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-κB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. The cells have been validated using purified human GITRL and anti-GITR neutralizing antibody.

Host Cell Line: Jurkat

Storage Stability: Store in liquid nitrogen immediately upon receipt.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• Artikelnummer: BPS60546
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60546
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human