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Nav1.8/β2 - HEK293 Recombinant Cell Line
Nav1.8/β2 - HEK293 Recombinant Cell Line
Artikelnummer
BPS60521
Verpackungseinheit
2 vials
Hersteller
BPS Bioscience
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Applications: Drug compound screening. Functional assays. Efficient antigen for mouse immunization
Background: Nav1.8 is a voltage gated type X, alpha subunit sodium channel which in humans is encoded by the SCN10A gene. It is expressed in nociceptors and has been proposed as a target for the development of new analgesics. Mice deficient in Nav1.8 have deficits in sensing inflammatory pain (initiated by tissue damage/inflammation) and visceral pain (initiated by damage or injury to internal organs) but not neuropathic pain. Native sodium channels are complexes composed of the pore-forming α subunit and an auxiliary β subunit. The sodium channel β2 subunit, encoded by the SCN2B gene, increases current amplitude and surface expression of sodium ion channels.
Description: Stable recombinant HEK293 cell line expressing tetracycline-inducible human Nav1.8 (Genbank #NP_006505.2) fused to Green Fluorescent Protein (GFP) [Ex. ~395 nm, 475 nm; em ~510 nm] and C-terminal Streptavidin-Binding Peptide (SBP)-tag, and the human sodium channel β2 subunit (Genbank #NM_004588; SCN2B) with C-terminal FLAG-tag. Nav1.8 is also known as tetrodotoxin-resistant voltage-gated sodium channel type X (SCN10A).
Format: Each vial contains ~1 X 106 cells in 10% DMSO solution
Genbank: NP_006505.2, NM_004588
Host Cell Line: HEK293
Mycoplasma Testing: The cell line is confirmed for absence of Mycoplasma species using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich).
Storage Stability: Immediately upon receipt, store in liquid nitrogen.
Tags: Nav 1.8: GFP and C-terminal Streptavidin-Binding Peptide (SBP)-tag. β2 subunit: C-terminal FLAG-tag.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Catterall, W.A. et al. Pharmacol. Rev. 57 (4): 397-409 (2005).
2. Muzny, D.M., et al. Nature 440:1194-1198 (2006).
3. Wilson, D.S., et al. Protein Expression and Purification 23 (3): 440-446 (2001).
Applications: Drug compound screening. Functional assays. Efficient antigen for mouse immunization
Background: Nav1.8 is a voltage gated type X, alpha subunit sodium channel which in humans is encoded by the SCN10A gene. It is expressed in nociceptors and has been proposed as a target for the development of new analgesics. Mice deficient in Nav1.8 have deficits in sensing inflammatory pain (initiated by tissue damage/inflammation) and visceral pain (initiated by damage or injury to internal organs) but not neuropathic pain. Native sodium channels are complexes composed of the pore-forming α subunit and an auxiliary β subunit. The sodium channel β2 subunit, encoded by the SCN2B gene, increases current amplitude and surface expression of sodium ion channels.
Description: Stable recombinant HEK293 cell line expressing tetracycline-inducible human Nav1.8 (Genbank #NP_006505.2) fused to Green Fluorescent Protein (GFP) [Ex. ~395 nm, 475 nm; em ~510 nm] and C-terminal Streptavidin-Binding Peptide (SBP)-tag, and the human sodium channel β2 subunit (Genbank #NM_004588; SCN2B) with C-terminal FLAG-tag. Nav1.8 is also known as tetrodotoxin-resistant voltage-gated sodium channel type X (SCN10A).
Format: Each vial contains ~1 X 106 cells in 10% DMSO solution
Genbank: NP_006505.2, NM_004588
Host Cell Line: HEK293
Mycoplasma Testing: The cell line is confirmed for absence of Mycoplasma species using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich).
Storage Stability: Immediately upon receipt, store in liquid nitrogen.
Tags: Nav 1.8: GFP and C-terminal Streptavidin-Binding Peptide (SBP)-tag. β2 subunit: C-terminal FLAG-tag.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Catterall, W.A. et al. Pharmacol. Rev. 57 (4): 397-409 (2005).
2. Muzny, D.M., et al. Nature 440:1194-1198 (2006).
3. Wilson, D.S., et al. Protein Expression and Purification 23 (3): 440-446 (2001).
| Artikelnummer | BPS60521 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60521 |
| Verpackungseinheit | 2 vials |
| Mengeneinheit | PAK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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