NFAT Luciferase Reporter CHO Cell Line (PKC/Ca2+ Signaling Pathway)

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Applications: Monitor NFAT stimulationScreen for activators or inhibitors of the PKC/ Ca²⁺

Background: The protein kinase C (PKC)/ Ca²⁺ response pathway leads to activation of the transcription factor nuclear factor of activator T cells (NFAT). NFAT is regulated by Ca²⁺ and the Ca²⁺/calmodulin-dependent serine phosphatase calcineurin. NFAT proteins are phosphorylated and reside in the cytoplasm in resting cells; upon stimulation, they are dephosphorylated by calcineurin, translocate to the nucleus, and induce gene expression.

Description: The NFAT Luciferase Reporter CHO cell line (PKC/Ca2+ Signaling Pathway) contains a firefly luciferase gene under the control of NFAT response element stably integrated into CHO K1 cells. This cell line is validated for its response to phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin.

Host Cell Line: CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. 

Supplied As: Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

• Artikelnummer: BPS78569
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78569
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Hamster