English
hERG (Kv11.1) - HEK293 Recombinant Cell line
hERG (Kv11.1) - HEK293 Recombinant Cell line
Artikelnummer
BPS60619
Verpackungseinheit
2 vials
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Background: hERG (human ether-a-go-go-related gene) encodes the alpha subunit of a potassium ion channel, Kv11.1. It contains six transmembrane α-helices with a re-entrant "poreloop" between the fifth and the sixth transmembrane helices. This ion channel is best known for its contribution to the electrical activity of the heart that coordinates the heart's beating. When this channel's ability to conduct electrical current across the cell membrane is inhibited or compromised, either by application of drugs or by raremutations, it can result in a potentially fatal disorder called long QT syndrome. A numberof clinically successful drugs in the market exhibit the potential to inhibit hERG, andcreate a concomitant risk of sudden death as a side-effect, which has made hERGinhibition an important off-target that must be avoided during drug development.
Biological Activity: The channel has been validated for activity using a fluorescence-based assay that usesthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye.
Description: Human ERG channel has been stably expressed in HEK293 cell line and its expressionwas confirmed by Western blotting. The channel activity of hERG was characterized by a fluorescence-based assay usingthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye. When hERG-HEK293 cells were pre-loaded withthallium-sensitive dye and stimulated with stimulus buffer containing potassium/thallium,thallium ions flowed through the open hERG channels into the cells and bound the dye,generating a fluorescent signal. The hERG channel activity in hERG-HEK293 cells wasblocked by hERG channel blockers, cisapride or dofetilide, causing the fluorescentsignal triggered by thallium influx to drop to the basal level.
Format: aqueous solution containing DMSO
Genbank: NM_000238
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM MycoplasmaDetection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
Supplied As: Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO
Biosafety Level: BSL-2
References: 1. Beacham, D.W. et al. (2010). J. Biomol. Screen. 15(4):441-446 2. Murphy, S.M. et al. (2006) J. Pharmacol. Toxicol. Methods. 54(1):42-55
Background: hERG (human ether-a-go-go-related gene) encodes the alpha subunit of a potassium ion channel, Kv11.1. It contains six transmembrane α-helices with a re-entrant "poreloop" between the fifth and the sixth transmembrane helices. This ion channel is best known for its contribution to the electrical activity of the heart that coordinates the heart's beating. When this channel's ability to conduct electrical current across the cell membrane is inhibited or compromised, either by application of drugs or by raremutations, it can result in a potentially fatal disorder called long QT syndrome. A numberof clinically successful drugs in the market exhibit the potential to inhibit hERG, andcreate a concomitant risk of sudden death as a side-effect, which has made hERGinhibition an important off-target that must be avoided during drug development.
Biological Activity: The channel has been validated for activity using a fluorescence-based assay that usesthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye.
Description: Human ERG channel has been stably expressed in HEK293 cell line and its expressionwas confirmed by Western blotting. The channel activity of hERG was characterized by a fluorescence-based assay usingthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye. When hERG-HEK293 cells were pre-loaded withthallium-sensitive dye and stimulated with stimulus buffer containing potassium/thallium,thallium ions flowed through the open hERG channels into the cells and bound the dye,generating a fluorescent signal. The hERG channel activity in hERG-HEK293 cells wasblocked by hERG channel blockers, cisapride or dofetilide, causing the fluorescentsignal triggered by thallium influx to drop to the basal level.
Format: aqueous solution containing DMSO
Genbank: NM_000238
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM MycoplasmaDetection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
Supplied As: Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO
Biosafety Level: BSL-2
References: 1. Beacham, D.W. et al. (2010). J. Biomol. Screen. 15(4):441-446 2. Murphy, S.M. et al. (2006) J. Pharmacol. Toxicol. Methods. 54(1):42-55
| Artikelnummer | BPS60619 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60619 |
| Verpackungseinheit | 2 vials |
| Mengeneinheit | PAK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
