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Application: Use as a negative control when testing PARG inhibitors in MCF7 cells.Cellular model for studies on the role of PARG.
Background: Poly (ADP-ribose) glycohydrolase (PARG) is a catabolic enzyme involved in the degradation of PARylated chains, releasing ADP-ribose and oligo (ADP-ribose) chains. PAR (poly-ADP ribosylation) homeostasis is regulated by the family of PAR polymerases (PARPs) and PARG in response to cellular stress conditions such as DNA damage response (DDR). PARG activity is linked to cellular responses in inflammation, ischemia, stroke, and cancer. PARG is overexpressed in breast cancer and associated with tumor growth and survival. Decrease in PARG activity can potentiate the effect of current cancer therapies, such as chemotherapy and radiation, making PARG inhibition with selective inhibitors a promising approach in cancer and immunotherapy.
Description: PARG Knockout MCF7 Cell Line is an MCF7 breast cancer cell line in which human PARG (Poly ADP-ribose glycohydrolase) has been genetically removed using CRISPR/Cas9 genome editing.This cell line has been validated by genomic sequencing and Western Blot.
Host Cell Line: MCF7 human breast mammary gland cell line. Adherent epithelial cells
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Uniprot: Q86W56
Warnings: Avoid freeze/thaw cyles
Biosafety Level: BSL-1
References: Marques M., et al., 2019 Oncogene 38 (12): 2177-2191.
James D. I., et al., 2016 ACS Chem Biol 11 (11): 3179-3190.
Drown B. S., et al., 2018 Cell Chem Bio 25 (12): 1562-1570.
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