HPBMC Peripheral Blood Mono, cryo amp

Lonzas Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood by apheresis and density gradient separation. Cells are collected from healthy donors following IRB protocols. They are guaranteed to contain ≥50 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC cell profiles differ by donors and can contain on average around 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. PBMCs of this part code have added characterization information available with population frequency values for T cells (CD3, CD4, CD8), B cells (CD19), Monocytes (CD14), NK cells (CD56), Dendritic cells (CD11c). With this information select the donor best suited to your experimental needs

Ability to Screen Different Donors
We maintain an inventory of different donors in an effort to satisfy your sample representation requirements. You can choose a lot based on donor demographic characteristics, and screen multiple lots to find the lot(s) that work best for you. In addition, we can request repeat donors if you need additional cells from a particular donor.

Rich Source of Immune Cells
Peripheral blood mononuclear cells (PBMC) are a mixed population of single nucleus cells. They can be further purified to isolate individual cell types such as natural killer cells, T cells, and B cells. In addition, PMBCs are a rich source of monocytes, which can be directed to differentiate into either macrophages or dendritic cells through culture with various cytokines. Researchers can choose X-Vivo 15 Hematopoeitic media that supports maintenance of lymphocytes and is optimized for serum-free culturing (additional cytokines may be necessary depending on application).

Benefits: Test negative for mycoplasma, bacteria, yeast, and fungi; HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots; Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications: T-cell Proliferation assays; Cytokine release assays; Macrophage polarization assay; Immunogenicity studies; Cell therapy process development; Humanized mice; Biomarker; discovery; Inflammatory studies; Immuno-oncology; CAR-T research

• Artikelnummer: LONCC-2702P
• Hersteller: Lonza
• Hersteller Artikelnummer: CC-2702P
• Verpackungseinheit: 1 AMP
• Mengeneinheit: STK
• Produktart: Cells