STAT3 Luciferase Reporter THP-1 Cell Line

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Applications: Monitor the STAT3 signaling pathway in a monocyte cell lineScreen for compound activity on the STAT3 signaling pathwayScreen for inhibitors of STAT3-mediated IL-6 signaling

Background: The JAK-STAT signaling pathway is involved in both cancer and autoimmune disease and has been an attractive target for drug discovery. In the context of immune regulation, STAT3 is expressed in diverse cell types. While it was initially described as an acute phase response factor in the context of IL-6 signaling, STAT3 has since been identified as a downstream regulator of many different cytokines. STAT3-mediated IL-6 signaling in macrophages has also been shown to play a role in their differentiation and proliferation. Studies of the JAK-STAT signaling pathways can prove useful in furthering our understanding of immunological diseases and cancer.

Description: The STAT3 Luciferase Reporter THP-1 cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Host Cell Line: THP-1, monocyte, suspension

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. 

Supplied As: Each vial contains ~2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Uniprot: P40763

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• Artikelnummer: BPS78498
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78498
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human