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TCF/LEF StemBright™ Luciferase iPS Cell Pool (Wnt Pathway)
TCF/LEF StemBright™ Luciferase iPS Cell Pool (Wnt Pathway)
Artikelnummer
BPS78515
Verpackungseinheit
1 vial
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Screen for Wnt-activating small molecules in iPS cells
Background: The Wnt signaling pathway is a well described regulator of organism development and cell fate specification. Cellular pluripotency and differentiation of multiple lineages, including cardiomyocytes and neurons are modulated by this pathway. There are 19 Wnt ligands and 10 Frizzled receptors, which stimulate canonical as well as non-canonical Wnt pathways. In the canonical pathway, cell activation by a Wnt ligand leads to the stabilization and nuclear translocation of β-catenin and subsequent activation of TCF/LEF transcription factor. The Wnt signaling pathway has recently been shown to play a role in cell cycle reentry and proliferation of iPS-derived cardiomyocytes. It is a therapeutic target in the treatment of neuropsychiatric disorders.
Description: The TCF/LEF (T-cell factor/lymphoid enhancing factor) StemBright™ Luciferase Cells contain a firefly luciferase gene under the control of TCF/LEF responsive elements, stably integrated into induced Pluripotent Stem (iPS) cells. TCF/LEF transcription factors are downstream of the Wnt signaling pathway. This cell pool is validated for its response to GSK3β inhibitor CHIR-99021, which activates the Wnt signaling pathway in human pluripotent stem cells.
Host Cell Line: Non-Disease Human iPS Cell Line (iXCells 30HU-002)
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. As this is a cell pool and not a cell line, BPS Bioscience cannot guarantee the stability of the genetic modifications over time. Clonal derivation may occur. We recommend freezing cell vials very early on and growing the cells for a limited number of passages. Cells should be cultured using Growth Media, which contain selection antibiotics.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of iPS cell freezing medium
Biosafety Level: BSL-2
Applications: Screen for Wnt-activating small molecules in iPS cells
Background: The Wnt signaling pathway is a well described regulator of organism development and cell fate specification. Cellular pluripotency and differentiation of multiple lineages, including cardiomyocytes and neurons are modulated by this pathway. There are 19 Wnt ligands and 10 Frizzled receptors, which stimulate canonical as well as non-canonical Wnt pathways. In the canonical pathway, cell activation by a Wnt ligand leads to the stabilization and nuclear translocation of β-catenin and subsequent activation of TCF/LEF transcription factor. The Wnt signaling pathway has recently been shown to play a role in cell cycle reentry and proliferation of iPS-derived cardiomyocytes. It is a therapeutic target in the treatment of neuropsychiatric disorders.
Description: The TCF/LEF (T-cell factor/lymphoid enhancing factor) StemBright™ Luciferase Cells contain a firefly luciferase gene under the control of TCF/LEF responsive elements, stably integrated into induced Pluripotent Stem (iPS) cells. TCF/LEF transcription factors are downstream of the Wnt signaling pathway. This cell pool is validated for its response to GSK3β inhibitor CHIR-99021, which activates the Wnt signaling pathway in human pluripotent stem cells.
Host Cell Line: Non-Disease Human iPS Cell Line (iXCells 30HU-002)
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. As this is a cell pool and not a cell line, BPS Bioscience cannot guarantee the stability of the genetic modifications over time. Clonal derivation may occur. We recommend freezing cell vials very early on and growing the cells for a limited number of passages. Cells should be cultured using Growth Media, which contain selection antibiotics.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of iPS cell freezing medium
Biosafety Level: BSL-2
| Artikelnummer | BPS78515 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 78515 |
| Verpackungseinheit | 1 vial |
| Mengeneinheit | STK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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