Product Type: Labelling Agent
CAS No.: 61135-33-9
MolecularFormula: C11H12N2O5
Molecular Weight: 252,2
Source: Synthetic
Purity: >98% (HPLC); NMR (Conforms)
Solubility: May be dissolved in DMSO (10 mg/ml)
Appearance: Off-white powder
PubChem CID: 472172
Scientific Background: 5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine that is used to monitor DNA synthesis through click chemistry (1). Unlike 5-bromo-2’-deoxyuridine (BrdU), EdU does not require sample fixation or DNA denaturation. It can then be detected using a fluorescent azide probe and copper catalysis using 1,3-dipolar cycloaddition.
References: 1. Salic A., and Mitchison, T.J. (2008) Proc. Natl. Acad. Sci. USA. 105:2415.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.